dhiya@alzahraa.edu.iq
RESEARCH INTERESTS
Published researchs on Scopus
Title: Berberine as a new neuraminidase inhibitor drug: A systematic review
Source Title: Journal of HerbMed Pharmacology
Abstract:
Influenza neuraminidase (NA) plays a main role in the viral replication of the influenza virus. It has been considered one of the targets for anti-influenza drugs. Anti-influenza drugs such as zanamivir, oseltamivir, and peramivir can fight the virus via the inhibition of NA. However, due to adverse reactions, the resistance of the viral strains and sudden changes in NA inhibitors, the identification of novel inhibitors is needed. Nature products such as berberine have been reported against influenza. In this systematic review, we have focused on the anti-influenza effects of berberine and its main role in the inhibition of NA of the virus. For this aim, “Berberine†was searched with “Influenza†or “flu†or “common cold†or “neuraminidase†in Web of Science, PubMed, Scopus, and Google Scholar databases from 1990 to April 2023. Studies have demonstrated that berberine and its derivatives have a wide range of biological effects such as antiviral effects against viruses like herpes simplex virus, human cytomegalovirus, and influenza A virus. The present study indicates that berberine and some of its derivatives are able to inhibit the influenza virus through NA blockade. Berberine is able to superimpose into the allosteric binding site and shows reversible noncompetitive behavior in the ligand-receptor interaction for the inhibition of NA. © 2024 Shahrekord University of Medical Sciences. All rights reserved.
Publisher: Shahrekord University of Medical Sciences
Title: Preparation and Evaluation of Buccal Adhesive Film Containing Smoke of Anbar Nesara (Donkey Dung) for Treatment of Recurrent Aphthous Stomatitis: Preparation a Randomized Controlled Clinical Trial
Source Title: Latin American Journal of Pharmacy
Abstract:
Recurrent aphthous stomatitis (RAS) is one of the most common ulcers of the oral mucosa that causes ulcers accompanied by pain and burning. Adhesive mucosal forms are a new drug delivery system that is available in tablet and film form. The film of buccal adhesive prolongs the stopping time of the drug, which leads to improved drug absorption. The purpose of this study, prepare and evaluate a buccal adhesive film containing smoke of Anbar Nesara was on the treatment of RAS. This study was performed as a double-blind randomized clinical trial on 80 patients. The study groups included the film group containing smoke and the other group the film without smoke. Buccal adhesive film of Anbar Nesara smoke was prepared using different compounds and appearance and pharmaceuticals properties, such as surface pH, adhesion strength and release time were investigated. Examination of films contained smoke showed that the prepared films had acceptable properties of appearance, surface pH and adhesion strength. Also, the prepared films containing smoke had significant therapeutic effects such as size decline, reduce inflammation and aphthous pain relief when applying the film contained smoke compared to control group. Smoke of Anbar Nesara have therapeutic effects on oral aphthous and lesions and buccal films are very effective as one of the new methods of drug delivery. © 2023, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Publisher: Colegio de Farmaceuticos de la Provincia de Buenos Aires
Title: Selegiline induced differentiation of rat bone marrow mesenchymal stem cells to dopaminergic neurons in vitro
Source Title: Pharmacia
Abstract:
Today, the use of mesenchymal stem cells (MSCs) for treating human diseases has attracted wide attention. The aim of this study is the expression of dopaminergic genes such as Nestin, patched Tumor Suppressor (PTCH), Sonic Hedgehog (SHH), Tyrosine Hydroxylase (TH) and Nuclear receptor-related factor 1 (NURR1) in MSCs after induction with selegiline. Rat bone marrow mesenchymal stem cells (rBMSCs) were extracted from femur and tibia bones and incubated with alpha Minimum Essential Medium (α-MEM) and 10% Fetal bovine serum (FBS). The stemness of cells at passage 4 was determined by the positive response to CD71 and CD90 markers and their differentiation into adipocytes and osteoblasts. The expression of SHH, PTCH, TH, NURR1 and Nestin genes in the cells after induction by 10-8 M selegiline for 48 hours was investigated by Reverse transcription polymerase chain reaction (RT-PCR) and Real Time-PCR methods. Isolated rBMSCs expressed CD71 and CD90 markers in culture conditions and could differentiate into adipocytes and osteoblasts. Induced cells showed neuronal morphology, positive response to Nestin and TH immunostaining. There was a significant increase of dopaminergic genes TH and NURR1 compared to the untreated cells. The results showed that selegiline with a dose of 10-8 M for 48 hours can lead to dopaminergic differentiation in rBMSCs. © Copyright Altememy D et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher: Pensoft Publishers
Title: Evaluation of berberine inhibitory effects on influenza neuraminidase enzyme: A molecular dynamics study
Source Title: Journal of HerbMed Pharmacology
Abstract:
Introduction: Due to the high prevalence and drug resistance reported for the influenza virus in recent years, much research is being conducted on the discovery and introduction of more effective drugs against the virus. In this regard, the present bioinformatics study examined the inhibitory effects of berberine, a plant-based alkaloid, on influenza virus neuraminidase using docking and molecular dynamics studies. Methods: To conduct this study, the three-dimensional structure and PDB file of influenza virus neuraminidase were prepared from the protein and molecular information database, and the structure file of the berberine and oseltamivir (as positive control) molecules were prepared from the PubChem database. Using GROMACS software, simulation and molecular dynamics calculations were performed in the absence of an inhibitor. Molecular docking studies were performed using AutoDock software, and re-simulation of the protein-ligand complex was performed using GROMACS software. Results: Berberine was bound to the neuraminidase molecule with three hydrogen bonds and eleven hydrophobic bonds at the binding site. The amount of binding energy (BE) of berberine and oseltamivir was equal to -7.93 and -6.27 kcal/mol with the estimated inhibition constant (EIC) of 1.5 and 25.2 μM, respectively. Over simulation time, the radius of gyration (Rg) of the enzyme at berberine binding increased, but there was no significant difference in system energy changes (TE). Conclusion: Due to berberine binding, structural changes occur in the secondary and tertiary structures of influenza virus neuraminidase. The large number of created bonds, the low level of binding energy, and the low concentration of the EIC indicate the high tendency of berberine to bind to the binding site of neuraminidase. © 2023 Nickan Research Institute. All rights reserved.
Publisher: Shahrekord University of Medical Sciences
Title: Determination of total antioxidant content of methanolic extracts of Cynara scolymus, Echinacea purpurea and Portulaca oleracea
Source Title: Advancements in Life Sciences
Abstract:
Background: The unique properties of medicinal plants particularly their effects on human health have attracted the attention of many researchers. The antioxidant properties of medicinal plants is a strong reason to use them in the food and pharmaceutical industries. Hence, the aim of this study was to evaluate the antioxidant properties of methanolic extracts of Cynara scolymus, Echinacea purpurea and Portulaca oleracea. Aerial parts of C. scolymus, E. purpurea and P. oleracea were dried and ground. Methods: Then, plant samples were prepared using homogenizing plant powders in methanol solution. Finally, the total antioxidant capacity of the plants was assessed by ferric iron reducing antioxidant power (FRAP) assay. Results: The results revealed that the total antioxidant capacity was found as 3.45, 1.16 and 1.68 mmol Fe2+/L for C. scolymus, E. purpurea and P. oleracea, respectively. Based on our results, C. scolymus, E. purpurea and P. oleracea showed a potent antioxidant activity. Conclusion: It is recommended that utilization of C. scolymus, E. purpurea and P. oleracea in food and pharmaceutical industries could possibly possess beneficial health effects. © 2022, The Running Line. All rights reserved.
Publisher: The Running Line
Title: Preparation of Transdermal Patch Containing Selenium Nanoparticles Loaded with Doxycycline and Evaluation of Skin Wound Healing in a Rat Model
Source Title: Pharmaceuticals
Abstract:
The present study aimed to prepare and evaluate a controlled-release system based on a chitosan scaffold containing selenium nanoparticles loaded with doxycycline. Its topical application in skin wound healing in rats was investigated. Therefore, 80 female rats were used and, after creating experimental skin defects on their back, were randomly divided into four equal groups: the control group without any therapeutic intervention; the second group received a chitosan transdermal patch (Ch); the third group received chitosan transdermal patch loaded with selenium nanoparticles (ChSeN), and the last group received chitosan transdermal patch containing selenium nanoparticle loaded by doxycycline (ChSeND). Morphological and structural characteristics of the synthesized patches were evaluated, and in addition to measuring the skin wound area on days 3, 7, and 21, a histopathological examination was performed. On the third day of the study, less hemorrhage and inflammation and more neo-vascularization were seen in the ChSeND group. Moreover, on day 7, less inflammation and collagen formation were recorded in the ChSeN and ChSeND groups than in the other groups. At the same time, more neo-vascularization and re-epithelialization were seen in the ChSeND group on days 7 and 21. In addition, on day 21 of the study, the most collagen formation was in this group. Examination of the wound area also showed that the lowest area belonged to the ChSeND group. The results showed that the simultaneous presence of selenium nanoparticles and doxycycline in the ChSeND group provided the best repair compared to the control, Ch and ChSeN groups. © 2022 by the authors.
Publisher: MDPI
Title: Improvement of Rat Cervicitis Using Medicinal Smoke Condensate of Jennet Feces: Animal Model set up and Vaginal Cream Production; Histopathological Study
Source Title: Journal of Pharmaceutical Negative Results
Abstract:
Introduction: Iranian traditional medicine has recommended the use of smoke from complete burning of jennet feces as a strong anti-inflammatory and wound healing component. Therefore, the present study was done to set up an animal model of cervicitis and production of vaginal cream of this smoke to evaluate anti-inflammatory effects of that in cervicitis. Materials and Methods: We used 20 female vistar rats weighting 200-250 gr in 4 groups for animal model set up using different dosage of phenol and CMC for the first time. The vaginal cream was containing 5% jennet faces smoke. Groups were divided in to health, cervicitis model, control (receive base cream) and treatment (receive smoke cream). After induction of cervicitis, control and treatment groups were received base cream and 5% vaginal smoke cream respectively for five days. Vaginal pH was measured during of study. After 5 days, the cervix organs were examined by histopathological assay. Result: No significant difference in vaginal pH in all of 4 groups of the intervention. In the model and control groups, we observed inflammatory cell infiltration and some visible epithelial erosion. After treatment with 5% vaginal smoke cream, the pathological features of cervicitis decreased significantly; inflammation (p = 0.011), vascular congestion (p = 0.001) and erosion scores (p> 0.005). Conclusion: The smoke of jennet feces has anti-inflammatory effects on phenol-CMC induced cervicitis in rat model. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Publisher: ResearchTrentz Academy Publishing Education Services
Title: Evaluation of Curcumin Effects on Bad, Bak, and Bim: A Molecular Dynamics Simulation Study
Source Title: Journal of Pharmaceutical Negative Results
Abstract:
Curcumin (Cur) is a polyphenol compound with antioxidant and apoptotic effects. It can lead cells to death which is the ultimate goal in cancer research. The effect of Cur on three pro-apoptotic factors of Bad, Bak, and Bim was investigated in this molecular dynamic and molecular docking simulation study. The PDB files of these three factors and the 3-D structure of Cur were obtained from www.rcsb.org and PubChem, respectively. All files were converted to PDB files by Avogadro v.1.2 software. The water and ion environment in GROMACS 2018 simulation package was used to conduct molecular dynamic simulation studies. Furthermore, AutoDock v.4.2.6 software performed the docking of Cur as a ligand and the proteins as receptors. LigPlot+ v.4.5.3 was used to determine the hydrogen and hydrophobic bonds at the binding sites. Results showed that Cur could bind to Bad with -6.58 kcal/mol binding energy. In addition, the binding of Cur to proteins induced some changes in molecular dynamic factors such as radius of gyration (Rg), root-mean-square deviation (RMSD), root-mean-square fluctuation (RMSF), binding energy (BE), estimated inhibition constant (EIC), and their secondary structures. A significant increase in Rg and RMSD and some variations in RMSF were seen in Bad and Bak after docking with Cur. Considering this study, Cur could bind to the proteins directly, induce conformational changes, and increase their likelihood of dimerization, leading to activating apoptotic pathways. These results suggested the apoptotic effects of Cur on cancer cells through influencing these proteins for the first time. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Publisher: ResearchTrentz Academy Publishing Education Services
Title: Current Medical Applications of Terminalia Chebula: An Overview
Source Title: Journal of Pharmaceutical Negative Results
Abstract:
Medicinal plants have long been used, and many of them are known today as drug production sources. The study and research of medicinal plants used in traditional medicine and their various effects in the treatment of some diseases can help produce new drugs. Terminalia chebula, known as the Haritaki plant, has always been at the top of Chinese, Tibetan, Ayurveda, and Iranian traditional medicines. Its classification is based on the size, shape, and growth of the fruit. We have done a thorough literature search in databases and collected the data and research results indexed in PubMed, Elsevier, and Institute for Scientific Information (ISI), Scientific Information Database, Google Scholar databases, and various books up to 2020. It has pharmacological uses such as neuroprotective, hepatoprotective, cardioprotective, antidiabetic, renoprotective, antifungal, antiviral, antibacterial, antimicrobial, antimutagenic, antiproliferative, antioxidant, antiarthritic, and antifertility properties. It also can act as an immunomodulator and anticancer agent. There are some available drugs obtained from T. chebula. Due to the different and wide pharmacological uses of Terminalia chebula, further studies are required to develop new drugs. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Publisher: ResearchTrentz Academy Publishing Education Services
Title: The Molecular Dynamics Effects of Rutin on CDKS 2, 4 and 6: In Silico Modelling and Molecular Dynamics
Source Title: Eurasian Journal of Medicine and Oncology
Abstract:
Objectives: This simulated study has mechanistically evaluated the molecular dynamics effects of rutin on CDKs 2, and 6 in cell cycling. Methods: Protein Data Bank (http://www.rcsb.org) was used to obtain the PDB file of CDK 2, 4, and 6. After simulation of CDKs in Gromacs software, AutoDock 4.2 was used to run 200 stages of molecular docking of CDKs in the presence of the rutin. CDK 2, 4, and 6 were simulated in the presence of rutin after docking. Results: Rutin had the highest tendency to bind the CDK-2 and CDK-6 via binding 16 and 18 residues in the binding site with hydrogen and hydrophobic bonds (respectively). Also, they had the highest amount of binding energy released. Rutin decreased total energy in CDKs and reduced the radius of gyration in CDK-2 and CDK-6 after docking. The second-ary coil structure increased in CDK-2 and decreased in CDK-4 and 6. Conclusion: Conformational changes in CDK2 and 6 via rutin can inhibit the activity of these proteins and subsequent-ly arrest the cell cycle in phases G1, S, and G2, which can lead the damaged cell to cell repair or Apoptosis. © 2022 by Eurasian Journal of Medicine and Oncology-Available online at www.ejmo.org.
Publisher: Kare Publishing
Title: The Restorative Effect of Titanium Dioxide Nanoparticles Synthesized with Origanum vulgare L., Carvacrol, Hypericum perforatum L., and Hypericin Loaded in Calcium Alginate Scaffold on Staphylococcus aureus-Infected Ulcers in Diabetic Rats
Source Title: Advances in Animal and Veterinary Sciences
Abstract:
In this study, the restorative effect of titanium dioxide nanoparticles (TiO2NPs) synthesized with Origanum vulgare, carvacrol, Hypericum perforatum, and calcium alginate scaffold-loadable calcium alginate on infected wounds with Staphylococcus aureus was investigated in diabetic rats. Eight groups of 12 rats in any group were diabetic by intraperitoneal injection of alloxan. After the rats were anesthetized, 2cm2 wounds were created and inoculated with bacterial suspension of S. aureus (ATCC 12600) equivalent to the 0.5 MacFarland tube. The hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum, and their active ingredients including carvacrol and hypericin, were prepared, and then TiO2N.Ps. were prepared from them using isopropoxide. The antibiotic methicillin and calcium alginate pad was also loaded as control of nanoparticles embedded in the calcium alginate pad and then the restorative effect on the wound was studied. On days 0, 3, 7, 14, and 21, hematoxylin-eosin slides (H&E) of the tissue, angiogenesis rate, and division of fibroblast cells and Masson’s trichrome slides were used to determine the rate of collagenization, wound, and extracellular matrix. After the treatment, the size of the wound in the Tio2 N.P. synthesis by Origanum vulgare, Tio2 N.P. synthesis by carvacrol, methicillin, Tio2 N.P., and alginate pad groups of diabetic rats and alginate pad group of non-diabetic rats were determined 7.03 ± 1, 11.12 ± 1, 10.72 ± 1 ± 15.48 ± 1, 11 ± 1, and 6.35 ± 1 mm, respectively. The serum level of TNFα in the Tio2 N.P. synthesis by Hypericum perforatum decreased from 62 ± 1 pg/mm on day 0 to 10 ± 1 pg/mm on day 21. Medicinal plants Origanum vulgare and Hypericum perforatum, especially their active ingredients hypericin and carvacrol, reduce wound microbial load and inflammation and ultimately repair wounds in diabetic rats due to antimicrobial and anti-inflammatory activities. They can therefore be used as an effective remedy for healing wound infections in diabetics. © 2022 by the authors. Licensee ResearchersLinks Ltd, England, UK. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons. org/licenses/by/4.0/).
Publisher: ResearchersLinks Ltd
Title: A comparative study of combination treatments in metastatic 4t1 cells: everolimus and 5-fluorouracil versus lithium chloride and 5-fluorouracil
Source Title: Pharmacia
Abstract:
Background: Combination therapy has been one of the most pioneering and strategic approaches implemented for malignancy treatment, which can intentionally influence multiple signaling pathways involved in cancer growth and progression. In the present study, the effects of 5-fluorouracil (5FU) in combination with everolimus (EVE) or lithium chloride (LiCl) were evaluated in 4T1 metastatic breast cancer cells and compared to control and each other. Methods and results: The resazurin assay, CompuSyn, flow cytometry, and real-time PCR were used to investigate cell proliferation, drug synergism, apoptosis, and gene expression. In comparison to the ternary combination of the drugs, the findings showed that cytotoxicity (p-value < 0.0001) and apoptosis (p-value < 0.0001) of two-by-two combinations increased dramatically as a consequence of the extreme synergy between 5FU and EVE or LiCl. Moreover, the hypoxiainducible transcription factor 1-alpha (HIF-1α) and the vascular endothelial growth factor (VEGF) downregulated considerably compared to control (p-value < 0.0001) by combination therapies of EVE-5FU and 5FU-LiCl; however, only VEGF displayed significant downregulation in comparison to single therapies. Conclusion: The findings showed that the combination of 5FU-LiCl increased cell cytotoxicity and apoptosis significantly more than EVE-5FU but suggests a clinical potential for both to treat metastatic breast cancer encouraging validation of these results in pre-clinical models. © Altememy D et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher: Pensoft Publishers
Title: Study of antibacterial effect of the hydroalchoholic extract of Teucrium polium against clinical strain of Gardnerella vaginalis in vitro
Source Title: Materials Today: Proceedings
Abstract:
Bacterial vaginosis is caused by a change in the dominant and normal flora of the vagina (lactobacilli) to a multimicrobial flora, including G. vaginalis. Today, due to the emergence of antibiotic resistance in pathogenic microbial species and side effects of antibiotic therapy, researchers have turned their attention to natural compounds. Teucrium polium (T.P) is a member of the mint family that has wide applications in traditional medicine and its antimicrobial effects have been shown in previous studies. The aim of this study was to evaluate the antibacterial effect of hydroalcoholic extract of T.P on the clinical strain of G. vaginalis in vitro. In the present experimental study, the hydroalcoholic extract of T.P was prepared by maceration method and total flavonoid content of T.P extract was determined. after preparing concentrations of 0.195–0.195 mg/ml, its antibacterial effect was determined by disk diffusion and macrobroth dilution methods. Data were analyzed by SPSS statistical software version 20. The flavonoid content of T.P extract was 8,033 mg routine equivalents per gram of dry extract. The MIC and MBC of the hydroalcoholic extract of T.P for G. vaginalis were 5 and 10 mg/ml respectively. The mean inhibition zone diameter at concentrations of 10, 5 and 2.5 mg/ml of hydroalcoholic extract of T.P were 12.67, 9.33 and 4.67 mm, respectively. In this study, it was found that the hydroalcoholic extract of T.P has in vitro antibacterial properties on the clinical strain of G. vaginalis and it is recommended for further study in clinical trials. © 2021
Publisher: Elsevier Ltd
Dhiya Raheem J. Altememy
Curriculum Vitae
Research Interests
Published researchs
الموقع الالكتروني