أ.د. زهير محمد علي جدوع الأسدي
السيرة الذاتية
الاهتمامات البحثية
البحوث المنشورة
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[email protected] : الايميل
لاسم: أ.د.زهير محمدعلي جدوع الاسدي اللقب العلمي : أستاذ المسيرة العلمية : البكالوريوس :الاختصاص العام : الطب والجراحة البيطرية (جامعة الموصل سنة 1990 – 1991) الماجستير : الاختصاص الدقيق : التكاثر والامراض التناسلية البيطرية(Theriogenology) (جامعة بغداد سنة 1994) الدكتوراه: / الهندسة الوراثية والتقنيات الاحيائية (جامعة بغداد سنة 2007) المسيرة الوظيفية : - 1996-1998 / تدريسي/ فرع العلوم الاساسية / كلية الطب البيطري - جامعة القادسية. - 1998-2003 / تدريسي / معهد المعلمين العالي / الزاوية / الجماهيرية العربية الليبية. - 2003-2006 / تدريسي ) اعادة تعيين / كلية الطب البيطري - جامعة القادسية . - 2009-2020 / تدريسي / كلية الطب/ جامعة كربلاء. -2007 -/ مقرر فرع التشريح واألنسجة والاجنة / كلية الطب / جامعة كربلاء. - 2007-2011 / رئيس فرع الاحياء المجهرية الطبية / كلية الطب – جامعة كربلاء. - 2008-2011 / معاون العميد للشؤون الادارية/ كلية الطب / جامعة كربلاء. - 2011-2019 / مساعد رئيس جامعة كربلاء للشؤون العلمية/ وكالة. - 2020 / تدريسي / جامعة الزهراء (عليها السلام) للبنات / كلية التقنيات الصحية و الطبية / اعارة خدمات من جامعة كربلاء. - 2020 / مساعد رئيس ا لجامعة للشؤون العلمية / جامعة الزهراء (عليها السالم ) للبنات . - 1991 / عضو نقابة الاطباء البيطريين والمسجل برقم التسلسل 4558. - خبرة تدريسية في مؤسسات التعليم العالي لمدة (27) سنة . عضوية الهيئة المهنية : 1- عضواللجنة التحضيرية للمؤتمرات الطبية السنوية/ كلية الطب / جامعة كربلاء. 2- رئيس لجنة الارشاد التربوي / كلية الطب / جامعة كربلاء / لعامي 2009 و 2010. 3- رئيس لجنة التقييم الذاتي / الاعتمادية / كلية الطب / جامعة كربلاء. 4- رئيس اللجنة العلمية / فرع الاحياء المجهرية / كلية الطب / جامعة كربلاء. 5- عضو لجنة اختبار صالحية التدريس / جامعة كربلاء. 6- رئيسا أو عضوا في العديد من اللجان العلمية و الادارية / كلية الطب / جامعة كربلاء. 7- نائب رئيس مجلس ضمان الجودة والاعتماد الاكاديمي في جامعة كربلاء. 8- رئيس لجنة الارشاد التربوي المركزية في جامعة كربلاء لسنة 2011/2010 . 9- رئيس اللجنة الامتحانية المركزية في جامعة كربلاء للاعوام الدراسية 2010 / 2011 , /2011 2012 , 2012/2013 , 2014/2013 , . 2019/2018 , 2018/2017 , 2017/2016 , 2016/2015 , 2015 /2014 10- عضو اللجنة الوزارية المركزية لمنح الاجازات الدراسية لمنتسبي الجامعات العراقية للعام الدراسي 2015/2014. 11- رئيس اللجنة المركزية لتداول المواد الكيمياوية والبايولوجية / جامعة كربلاء للفترة من 2012 الى .2019. 12- رئيس لجنة تصنيف الجامعات حسب المعايير الوطنية / جامعة كربلاء.
الوراثة الجزيئية الطبية ، البايولوجي الجزيئي ، التشخيص الجزيئي للأمراض الوراثية ، التقنيات الجزيئية المتقدمة، تطبيقات الهندسة الوراثية والعلاج الجينيِ
Immunological Evaluation of Patients with Β-Thalassemia Major in Kerbala City Using Single Radial Immunodiffusion (SRID) Techniqu
Beta-thalassemia major is one of the major health problems in our country. Many studies have confirmed the fact that, these patients have an increased susceptibility to bacterial infections, assumed to be the result of immunological changes.
Improved eco-friendly CsSn 0.5 Ge 0.5 I 3 perovskite photovoltaic efficiency beyond 20% with SMe-TATPyr hole-transporting layer
الوصف Perovskites composed of inorganic cesium (Cs) halide provide a route to thermally resistant solar cells. Nevertheless, the use of hole-transporting layers (HTLs) with hydrophobic additives is constrained by moisture-induced phase deterioration. Due to significant electrical loss, dopant-free HTLs are unable to produce practical solar cells. In this article, we designed a two-dimensional 1,3,6,8-tetrakis[5-(N,N-di(p-(methylthio)phenyl)amino-p-phenyl)-thiophen-2-yl]pyrene (termed SMe-TATPyr) molecule as a new HTL to regulate electrical loss in lead-free perovskite solar cells (PSCs). We optimized the power conversion efficiency (PCE) of PSCs based on mixed tin (Sn)/germanium (Ge) halide perovskite (CsSn0.5Ge0.5I3) by exploring different factors, such as the deep and shallow levels of defects, density of states at the valence band (NV), thickness of the perovskite film, p-type doping concentration (NA) of HTL, the
Molecular detection and discrimination of three poultry Eimeria species in Kerbala and Babylon provinces, Iraq
Coccidiosis is an intestinal disease of chickens in which the Causes include a various species of protozoan parasites that located within the genus Eimeria. Diagnosis and genetic characterization of different species of Eimeria are central dogma to the preventative, resistance, and control of coccidiosis. The aim of the present study was to identified different avian Eimeria species that considered the causal agents of avian coccidiosis in each of the Kerbala and Babylon provinces, Iraq. The present study observations were revealed increasing and decreasing the percentages of infection with coccidiosis during different months of a year and that due to the large change in temperature and humidity in Iraq, so the results showed that the highest percentage of infection in both of Kerbala and Babylon provinces were in December (23.12%) and the lowest percentage in July (1.87%
The association of vascular endothelial growth factor polymorphism (rs699947) with diabetic foot ulcer and oxidative status
Diabetic foot ulcer (DFU) is a common, complex, and costly complication of diabetes that affects the lower extremities. Foot ulceration is the most frequently recognized issues in this disease. The vascular endothelial growth factor (VEGF), encompassing 14 kb, is located on chromosome 6p21.3, and contains 8 exons and 7 introns. There are at least 25 different single nucleotide polymorphisms (SNPs) in the VEGF that are associated with DFU, the current study deals with the most frequent and important one, the rs699947.
Synthesis and characterization of fullerene modified with copper nanoparticles catalyzed CH bond activation as a sustainable and green catalyst for electro synthesis of
In this study, our focus was on synthesizing and characterizing a fullerene modified with copper nanoparticles. This modified fullerene catalyzes for the eco-friendly synthesis of benzothiazole 5(a-m) derivatives. These derivatives are obtained through the reaction of 2-iodoaniline 1(a-f), toluene 2(a-e), and Na2S resulting in good yields (89–94 %). The nanocomposite catalyst underwent thorough characterization using various analytical techniques, including Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDS), Brunauer–Emmett–Teller (BET), Thermogravimetric analysis (TGA), Ultraviolet (UV), X-ray photoelectron spectroscopy (XPS), Cyclic voltammetry (CVs) and X Ray Diffraction Analysis (XRD) spectroscopy. By utilizing this catalyst, we achieve a more environmentally friendly approach to synthesis, reducing the requirement for …
Morphology analysis and microbial diversity in novel anaerobic baffled reactor treating recycled paper mill wastewater
The profile of microbial diversity in a NABR digesting RPMW was investigated using phylogenetic analysis of partial 16S rRNA sequences by a neighbor-joining-tree, supported by microbial morphology analysis by SEM. The results showed that microorganism inside NABR consisted of dominant Bacillus (25 strains) and Bacterium (1 strain) which were isolated from the settled sludge at the bottom of the reactor, whilst Bacillus (2 strains), Pseudomonas (2 strain) and Chryseobacterium (2 strain) were isolated from the biofilm formed on the packing material. It revealed that the microbial community strains, function, and structure changed simultaneously throughout the reactor system. The microscopic results showed rich biofacies, while the dominant microorganisms have various morphologies in every compartment of the system. It consisted of a long rod-shaped and filamentous bacterium composed majorly of bacilli of different sizes. Although the study successfully analyzed the microbial diversity and morphology in the system, the microbial communities reported in this study were different from other similar studies. This may be caused by the application of a culture-based technique that usually provides limited information due to the number of barely cultivated or uncultured strains.
Molecular Detection of MTHFR gene Polymorphisms in Transitional Urinary Bladder Cancer Patients
The amplified MTHFR gene PCR products underwent restriction enzyme digestion for 60 min at 37 C. They were separated using 2% agarose gel electrophoresis and visualized with UV light. Molecular Detection Using Determination of Sequencing DNA Sequencing MTHFR A1298C polymorphism Figure 4 shows the sequence of multiple sequences of the MTHFR A1298C reference gene with all four selected samples where samples A, B and C samples of bladder cancer patients and sample D represented the control sample. The results were analyzed using the Bio Edit Sequence Alignment Editor Software, the analysis result showed the presence of one SNP (C66T) in the four samples Mutations in the multilevel MTHFR gene C677T and A1298C cause a decrease in the level of folate and an increase in homocysteine, which causes many diseases (15). Polymorphisms of methylenetetrahydrofolate reductase …
Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows
The present study was carried out to investigate the possible etiology and pathology of cystic ovarian follicles by determining the expression levels of mRNA for LH-r gene in follicular cells of dominant follicles in comparison with cystic ovarian follicles in Iraqi cows. This study were performed in two steps, firstly aspiration of follicular fluids from dominant follicles and cystic follicles and stored in (-20℃) until estradiol and progesterone assay, secondary section of the follicular wall in to two hemispheres, and stored at-70℃ to-80℃ to molecular study. The macroscopic examination of the ovaries revealed that the numbers of dominant follicles are [1] samples while cystic follicles were in [2] samples. There was a significant difference (P< 0.05) in the diameter of cystic ovarian follicles (37.56±0.64 mm) compared with dominant follicles (19.93±0.32 mm). Results of hormonal assay showed higher estradiol-17β (865.96±10.64 ng/ml) and progesterone (84.8±1.35 ng/ml) concentrations in follicular fluids of cystic ovarian follicles, which were significantly higher (P< 0.05) in comparison with those of dominant follicles which were (314.39±2.55 ng/ml) and (50.25±1.57 ng/ml) respectively.
Evaluation of (HPLC) patterns of sickle cell anemia patients in comparison with apparently healthy individuals
Objectives: 1-This study aimed to evaluate the chromatographic pattern of Hb types (HbA, HbF, HbA2 and HbS) of sickle cell anemia patients in comparison with the apparently healthy individuals.
Prevalence of coccidiosis in broiler-chicken farms in the Kerbala and Babylon provinces, Iraq.
Coccidiosis is an intestinal disease of chickens caused by various species of protozoan parasites within the genus Eimeria. Diagnosis and genetic characterization of different species of Eimeria are central to the prevention, surveillance, and control of coccidiosis. The aim of the present study was to be identified different poultry Eimeria species that considered the causal agents of poultry coccidiosis in each of the Kerbala and Babylon provinces.
ASSOCIATION OF MDM2 (T309G) GENE POLYMORPHISM WITH INTERSTITIAL URINARY BLADDER CANCER
The importance of the current study in the investigation of polymorphism in MDM2 gene, which is one of the important genes with formal variations that have a prominent role in the incidence of cancer of the Transitional urinary bladder, where the study of polymorphisms helps distinguish between the different inherited forms of the gene has humans have a large number of genetic polymorphisms in DNA. The study samples were collected from 60 patients after being diagnosed clinically and histologically by the specialist surgeon of the Imam Hussein Center for Cancer and Hematology in Al Hussein Hospital and Al Safir Hospital in the holy governorate of Karbala, Iraq, in addition to 40 healthy individuals. DNA was extracted from blood samples for all study samples. Molecular detection of single nucleotide polymorphisms (SNPs) was carried out at the genetic sites of MDM2 gene using PCR-RFLP technique …
haematological study of β-thalassaemia among iraqi population
Blood samples of 80 clinically thalassaemic patients were collected from three thalassaemic centres in Iraq, in addition to 56 apparently healthy individuals were collected as a control group. Haematological examinations were applied for some parameters. The blood groups were determined for all samples, the relationships between blood groups and both gender and phenotypes of β-thalassaemia were studied. The results showed that there were no significant relationship at (p> 0.05) between blood groups and both parameters (gender and phenotypes) for patients in comparison with the control group. The estimations of Hb concentrations, quantitative and qualitative Hb analyses by Hb electrophoresis in order to determine the Hb types (HbF, HbA, and HbA2) and reticulocyte count, were adopted for 31 βthalassaemic patients and 20 apparently healthy individuals. According to the haematological examinations data, the relationships of gender, blood groups, and phenotypes with Hb concentrations, Hb types, and reticulocyte count were studied.
Molecular Detection of Vancomycin Resistance Genes in Staph aureus Isolates from Different Clinical Specimens
The study screened the occurrence of VanA, VanB, Vanc genes in Staph aureus vancomycin-resistant isolates recovered in Hilla-hospitals. From various clinical samples, 160 isolates from patients between July and November of 2022 were included in the search. Among these samples are (65) burn samples,(55) urine samples, and (40) samples from Blood. 40 (or 25%) of the 160 isolates were Staph aureus. Characterization of the VanA, VanB, and VanC genes in Staph aureus isolates obtained from various specimens is the main goal of the work. The RESULTS of samples were obtained from a variety of clinical specimens, including 20 (40.625%) burn swabs, 10 (25%) urine samples, and 10 (34.375%) blood. Staph aureus was resistant to Cefepime (91.7%), Piperacillin (73.3%), and Ceftazidime (71.7%), although little relationship was detected with Aztreonam resistance (35%). 40 isolates were used for antibiotic sensitivity test (AST). Only 18.3% and 15% of isolates, respectively, were resistant to meropenem and imipenem. Fluoroquinolone resistance to lomefloxacin, ciprofloxacin, ofloxacin, and levofloxacin was 51.7%, 48.3%, 35%), and 30%, respectively. While the most prevalent vancomycin resistance genes were found in 40 isolates of S. aureus and were discovered by VanC 40 (100%), VanA 20 (100%), and VanB28 (70%), according to the current investigation. In Conclusion: The PCR-reaction method was discovered to be an easy-to-use tool for detecting staph aureus by pcr that can be regularly used for identifying staph aureus and detection the molecular resistance apparatuses to vancomycin in staph aureus by VanA, VanB, VanC …
Evaluation of the hematological and immunological markers after the first and second doses of BNT162b2 mRNA vaccine.
OBJECTIVE: Both humoral and cellular immunity can be significantly influenced by the immunological responses to vaccination, and both responses are essential. Vaccination is the most consistent, safe, and cost-efficient practice for controlling the COVID-19 pandemic. PATIENTS AND METHODS: Blood samples were collected from participants who received two vaccine doses of COVID-19 Pfizer/BioNTech (BNT162b2) before and on days 7 and 10 after the first and second immunization. We evaluated some hematological and immunological markers responses to the 1st and 2nd doses of the BNT162b2 mRNA (Pfizer/BioNtech) vaccine. RESULTS: In healthy subjects’ neutrophil and WBC counts significantly increased compared to those after the first dose. The results of all first-group participant categories demonstrated no discernible variations in lymphocyte counts. There was no change in IgM or IgG in all second-group cohorts, except for a considerable rise in IgG levels in people with a history of coronavirus infection following the second dosage compared to baseline. After the second dose, CD4+ T-cell and CD8+ T-cell levels rose in all groups compared to before the immunization and after the first dosage. Data demonstrated a substantial rise in neutrophil-lymphocyte ratio (NLR) after the second dose of the vaccine. Individuals who had previously had COVID-19 disease experienced a considerable increase in C3 and C4 levels after the first and second dosages compared to baseline. Additionally, compared to their levels after the first dosage, C4 levels increased significantly following the second dosage. Interleukin (IL)-6, IL-15 …
First Detection of Cucurbit yellow stunting disorder virus in Iraq
During the growing season of 2022, courgetti plants displaying foliar yellowing that included leaf mottle and interveinal chlorosis along with green veins were observed in numerous courgetti fields of Al-Yusufiyah, Baghdad Province, Iraq. Bemisia tabaci was abundant with the foliar yellowing plants in all fields investigated. Fifty symptomatic leaf samples showing typical foliar yellowing were collected randomly from several courgetti fields. Three representative samples were sent to Macrogen (Seoul, South Korea) for high-throughput RNA sequencing. The BLASTn analyses of the assembled genome of cucurbit yellow stunting disorder virus (CYSDV) isolate Iraq-1 segment RNA1 and RNA2 demonstrated pairwise nucleotide identity between 99.23 and 99.84% for segment RNA1 and between 97.58 and 97.92% for segment RNA2 with several corresponding global isolates of CYSDV. These two segments of the …
First Report of Cockscomb Root Rot Caused by Fusarium solani in Iraq
Cockscomb (Celosia argentea L.) is one of the most important ornamental plants in Iraq. In August 2021, severe root rot of cockscomb plants was observed, with an incidence of 30%, in all of the nurseries surveyed in the Karbala and Babylon provinces, Iraq. The symptoms appeared as severe leaf chlorosis and wilting with brown discoloration of the stem vascular bundle associated with dark brown lesions on the root that extended to the root collar and became extensive rot. The morphological features of 50% of the fungal colonies were identical and similar to those of Fusarium spp. The internal transcribed spacer, translation elongation factor, RNA polymerase I and II, beta-tubulin 1, and mitochondrial small-subunit ribosomal RNA genes were amplified and sequenced. BLASTn analysis of these genetic markers’ sequences found 99.84 to 100% similarity with the corresponding global sequences of Fusarium solani …
Toll-like receptor 4 gene polymorphism of patients with urinary tract infection in Karbala province, Iraq
Urinary Tract Infections are the most common bacterial infections. TLR4 gene has several polymorphisms that are strongly associated with rising susceptibility to get infection with gram-negative bacteria. The present study aimed to investigate the association between single nucleotide polymorphisms (SNPs)(T1196C),(A896G) in the toll-like receptor 4 (TLR4) genes and the incidence of Urinary Tract Infection. A total of 50 patients with Urinary Tract Infection and 30 healthy control enrolled in this study. Blood samples from patients with UTI and control were collected at AL-Imam AL-Hussain teaching hospital in Karbala province during the period from January to October 2020. DNA was extracted from the blood samples of patients and healthy control. The target region of TLR4 genes were amplified with polymerase chain reaction (PCR) using specific primers. The study showed a significant association between the
Human leukocyte antigen-DQA1* 0501 allele and its association with pathogenesis of type 1 diabetes mellitus among Iraqi childrenhttps://www.sciencedirect.com/science/article/pii/S2452014419301591
Type 1 diabetes mellitus (T1DM) is an autoimmune disease, arising through a complex interaction of both genetic and immunologic factors. There is an association between human leukocyte antigen (HLA) complex and T1DM in various populations. The allele of HLA-DQA1 gene polymorphism associated with T1DM is HLA-DQA1*0501. The blood glycated hemoglobin (HbA1c) level provides the average blood glucose levels during two to three months, which is the predicted half-life of red blood cells (RBCs).
Association of Adiponectin Gene Promoter Polymprphism (rs266729) With Ischemic Heart Diseases
background: Ischemic Heart Disease is a group of diseases of the blood vessels supplying the heart muscle. Adiponectin is a protein secreted by adipocyte with insulin-sensitizing, Anti-inflammatory and anti-atherogenic properties. Several studies have shown that polymorphisms within the adiponectin gene can be associated with Ischemic heart disease. Aim: The aim of the present study was to evaluate the impact of (rs266729) SNP in the promoter region of the ADIPOQ gene on the occurrence of Ischemic heart disease (IHD). Methods: The study included 150 patients with IHD randomly selected based on World Health Organization (WHO) guideline and 150 as controls group. DNA was extracted from blood and genotyped by PCR-RFLP by using (Hha1) enzyme. Result: The frequency of G allele of rs266729 (C/G) polymorphism was significantly (p= 0.0001) in IHD (19.6%) compared with control (13.3%). The …
Association of Adiponectin Gene Promoter Polymprphism (rs266729) With Ischemic Heart Diseases.
Abstract Background: Ischemic Heart Disease is a group of diseases of the blood vessels supplying the heart muscle. Adiponectin is a protein secreted by adipocyte with insulinsensitizing, Anti-inflammatory and anti-atherogenic properties. Several studies have shown that polymorphisms within the adiponectin gene can be associated with Ischemic heart disease. Aim: The aim of the present study was to evaluate the impact of (rs266729) SNP in the promoter region of the ADIPOQ gene on the occurrence of Ischemic heart disease (IHD). Methods: The study included 150 patients with IHD randomly selected based on World Health Organization (WHO) guideline and 150 as controls group. DNA was extracted from blood and genotyped by PCR-RFLP by using (Hha1) enzyme. Result: The frequency of G allele of rs266729 (C/G) polymorphism was significantly (p= 0.0001) in IHD (19.6%) compared with control (13.3 …
Molecular characterization of Beta-thalassemia Mutations in Holy Karbala
AbstractBeta-thalassemia is autosomal recessive disease that spread inhigh percentages around the world. the importance of the presentstudy was in evaluating the disease in holy Kerbala، Iraq using moleculartechniques. A total of 36 of blood samples were collected from beta-thalassemiamajor patients، 20 samples intermedia thalassemic patients fromthalassemia center-AL-Hussain medical city in holy Kerbalagovernorate، and 20 samples from apparently healthy individuals as acontrol group. The DNA molecules were extracted from all samples for molecularcharacterization of four β-thalassemia mutations are [IVS1-110 (G> C)، CD8/9 (+ G)،-87 (C> G)، CD41/42 (-TCTT)] using PCR basedtechnique called Amplification Refractory Mutation System (ARMS). The results revealed that IVS1-110 is the common mutation70%) in the studied samples، the CD8/9 (+ G) mutation was) 14diagnosed for first time in holy Kerbala 2 (10%)، the-87 (C> G) wasdiagnosed for the first time in Iraq in percentage 4 (20%) and finally theCD 41/42 was not diagnosed in the studied samples. Geographical distribution of diagnosed mutations were studied thedistribution of mutations within Kerbala regions، the results showedthat the city center was more affected with diagnosed mutations. Finally، results also revealed that heterozygotes were the moregenotypic patterns 20 (74.07%) of mutations in comparison withhomozygotes with were 5 (18.52%)، and the compound cases were2 (7.41%) of studied mutations. Key words: Thalassemia، ARMS-PCR، Mutations، IVS1-110 (G> C)، CD8/9 (+ G)،-87 (C> G)، CD41/42 (-TCTT)، Kerbala.
The Role of Tumor Markers in Early Detection of Brest Cancer in kerbala Governance
In many cases of cancer, the tumor markers play an important role in early detection of the diseases. Blood sample of (69) breast cancer patients were collected from early detection unit in AL-Hussein teaching hospital in Kerbala governorate, which compared with (30) apparently healthy women. The CA27. 29 tumor marker was adopted for detection of breast cancer, the statistical analysis revealed there is highly significant correlation (P≤ 0.01 (between the tumor marker and both types of breast cancer in comparison to the control group, the results also showed no significant effects (P≥ 0.05) for the (age, marital status, breast feeding, number of children, weight, parent relationship)
Effect of Dimethylsulfoxide and Betaine on duplex polymerase chain reaction of human beta-globin gene amplification
A variety of additives and enhancing agents can be included in PCR amplifications to increase yield, specificity and consistency of PCR products. Blood samples were collected from (35) apparently healthy individual for DNA extraction and duplex PCR amplification according to the four different PCR component set up include: 1-standard set up (without PCR additives), 2-standard PCR set up plus 5% DMSO, 3-standard PCR set up plus 1M betaine, and 4-standard PCR set up plus 5% DMSO and 1M betaine. The results revealed that the standard optimization condition of duplex PCR amplification is enough to reveal a good PCR amplification of human beta-globin gene, and the studied PCR additives are useful in improvement of amplification in combination of 5% DMSO and 1 M betaine.
Molecular characterization of some mutations in BRCA1 and BRCA2 genes of breast cancer patients
The importance of this study is the investigation of mutations in BRCA1 and BRCA2 genes as tumor suppressor genes related to occurrence of breast cancer, and its correlations with tumor marker. Blood sample of (69) breast cancer patients were collected from early detection unit in AL-Hussein teaching hospital in Kerbala governorate, which compared with (30) apparently healthy women. The CA27. 29 tumor marker was adopted for detection of breast cancer, The DNA was extracted from blood samples, the detection of BRCA1 and BRCA2 gene mutations were adopted using PCR technique, the molecular diagnosis for (185 del AG) and (5382 ins C) mutations in BRCA1 gene and (6174 del T) mutation BRCA2 gene were applied. The results showed that the higher percentage of mutations were (27.5%) for (185 del AG) mutation in sporadic breast cancer group, and for hereditary breast cancer group, the percentage of (185 del AG) mutation was (20%) and for (5382 ins C) mutation was (10%), while the (6174 del T) mutation is not recorded in both patient groups. And the results also revealed that the total percentages of (185 del AG),(5382 ins C) and (6174 del T) for patient group were (23.19%),(5.8%) and (0%) respectively, and there were no mutation detection in control group. Finally, the results showed there were no correlation between the tumor marker and mutations, and highly significant correlations between tumor marker of patients in comparison with control group. Key words: Breast cancer, PCR, Mutations, BRCA1, BRCA2, Kerbala
Effect of Dimethylsulfoxide and Betaine on duplex polymerase chain reaction of human beta-globin gene amplification
A variety of additives and enhancing agents can be included in PCR amplifications to increase yield, specificity and consistency of PCR products. Blood samples were collected from (35) apparently healthy individual for DNA extraction and duplex PCR amplification according to the four different PCR component set up include: 1-standard set up (without PCR additives), 2-standard PCR set up plus 5% DMSO, 3-standard PCR set up plus 1M betaine, and 4-standard PCR set up plus 5% DMSO and 1M betaine. The results revealed that the standard optimization condition of duplex PCR amplification is enough to reveal a good PCR amplification of human beta-globin gene, and the studied PCR additives are useful in improvement of amplification in combination of 5% DMSO and 1 M betaine.
Immunological Evaluation of Patients with Β-Thalassemia Major in Kerbala City Using Single Radial Immunodiffusion (SRID) Techniqu
background: Beta-thalassemia major is one of the major health problems in our country. Many studies have confirmed the fact that, these patients have an increased susceptibility to bacterial infections, assumed to be the result of immunological changes. Objective: This study aimed to evaluate some of humoral immunological parameters of thalassemic patients by measuring their serum concentration of Immunoglobulin (IgG), IgM, IgA, and serum complement components C3 and C4. Patients & Methods: A total of forty five beta-thalassemia major patients who were attending to the thalassemia center in Kerbala teaching pediatrics hospital (24 males and 21 females) from January to August of 2009 and fifty of control children with matched age and
Evaluation of (HPLC) Patterns of Sickle Cell Anaemia Patients in Comparison with Apparently Healthy Individuals
background: Sickle cell haemoglobin (HbS) results from an autosomal recessively inherited mutation in which the amino-acid glutamine is replaced by valine at position 6 in the beta globin chain of haemoglobin (Hb). Sickle cells have a reduced deformability and are easily destroyed, causing occlusion of the microcirculation and a chronic haemolytic anaemia with a median Hb concentration level of about 9 g/dl. Routine electrophoresis methods and High performance liquid chromatography (HPLC) were used to screen normal and variant Hb, and allowed the verification of the Hb observed with electrophoresis and precise quantification of their proportion. Objectives: 1-This study aimed to evaluate the chromatographic pattern of Hb types (HbA, HbF, HbA2 and HbS) of sickle cell anemia patients in comparison with the apparently healthy individuals.
Molecular Detection of Some Mutations Associated with Βeta-Thalassaemia in Iraq
the study was carried out in period between “1 August 2005 to 30 of December 2006”. Blood samples of 80 clinically thalassaemic patients were collected from three thalassaemiacenters in Iraq, namely: Ibn Albalady central thalassaemia center in Baghdad, and also fromKerbalaa and Al-Qadissya governorates. Blood samples were also collected from 56 apparentlyhealthy individuals to serve as a control group. DNA was isolated from blood and used for molecular detection of seven types of β-thalassaemiamutations (IVS1 nt. 1 G--A, IVS1nt. 5 G--C, IVS1 nt. 6 T--C, IVS1 nt. 110 G--A, codon 39 C--T, IVS2 nt. 1G--A, and IVS2 nt. 745 C--G) using the PCR based technique called ARMS (Amplification Refractory Mutation System). Five out of seven of these diagnosed mutations were reported for the first time in Iraq, and themost frequent β-thalassaemia mutations were codon 39 and IVS1 nt. 110 with the proportions (26.76%) and (20.34%), respectively. No IVS2nt. 745 was detected within the studied samples. Genotypic distribution of the samples indicated that there is no significant difference (p> 0.05) between the frequency of homozygotes and heterozygotes within patient group, while there is asignificant difference at (P< 0.01) in comparison with the control group. The study of association between the number and the types of mutations revealed that 28 (58%) ofpositive cases have single mutation in a homozygous state or heterozygous state whichsignificantly associated at (P< 0.05) with β-thalassaemia mutations, whereas 20 (42%) of thesecases have compound mutations. The most frequent association appeared between IVS1 nt. 110 andCodon …
Original Paper Association of Adiponectin Gene Promoter Polymprphism (rs266729) With Ischemic Heart Diseases
Aim: The aim of the present study was to evaluate the impact of (rs266729) SNP in the promoter region of the ADIPOQ gene on the occurrence of Ischemic heart disease (IHD). Methods: The study included 150 patients with IHD randomly selected based on World Health Organization (WHO) guideline and 150 as controls group. DNA was extracted from blood and genotyped by PCR-RFLP by using (Hha1) enzyme. Result: The frequency of G allele of rs266729 (C/G) polymorphism was significantly (p= 0.0001) in IHD (19.6%) compared with control (13.3%). The homozygous genotype (GG) significantly (0R= 1.71, CI 95%= 0.65-4.96, P= 0.0001) increased the risk of Ischemic Heart Disease compared with wild type (CC) after adjustment age, sex, and BMI, furthermore the heterozygous (CG) genotype significantly (0R= 1.61, CI 95%= 0.96-2.87, P= 0.0001) raised the risk of Ischemic Heart Disease. Conclusion: Adiponectin gene polymorphism rs266729 is involved in the pathogenesis Ischemic heart disease.
A Computational Effort to Untangling Anti-Sars-Cov-2 Effects of Oleanolic Acid Analogues
Considering the current global pandemic and the urgent need to introduce novel drug candidates against severe acute respiratory syndrome corona virus 2 (SARS-CoV-2), we reported at least four antipyretic recipes of Kurdish ethnomedicine which can be translated to functional antiviral formulations in orthodox medicine. Our current demanding computational work places much emphasis upon the implications of oleanolic acid and its analogues as a cluster of binder candidates of the main protease (Mpro) of SARS-CoV-2 which having a pivotal role in the pathogenesis of coronavirus disease 2019 (COVID-19). Through molecular docking and simulation studies, we found oleanolic acid (-12.6 Kcal/mol) and its two analogues (OA11; ligand I (-14.2 Kcal/mol)) and (OA31; ligand II (-14.0 Kcal/mol)) bound with Mpro (PDB: 6Y84) more reliable and trustful than saquinavir (-8.1 Kcal/mol) as a canonical drug.[2] Salaspermic acid,(3b)-3-{[(2E)-3-phenylprop-2-enoyl] oxy} olean-12-en-28-oic acid, OA37 and OA40 interacted with catalytic dyad and major amino acid residues of active sites of Mpro and these toxic compounds should be considered in future anti-protease drug design. Overall, the current study seized the attention of experimentalists to the new set of anti-protease pentacyclic triterpenoids that should to be assayed against SARS-CoV-2 at invitro or in clinical settings of COVID-19.MOLECULAR BASIS OF HUMAN LEUKOCYTE ANTIGENS DQB1* 0201 ALLELE AND ITS ASSOCIATION WITH TYPE 1 DIABETES MELLITUS OF IRAQI CHILDREN
Type 1 diabetes mellitus (T1DM) is an autoimmune disease arising through a complex interaction of both genetic and immunologic factors. Similar to the majority of autoimmune diseases, T1DM usually has a relapsing remitting disease course with autoantibody and T cellular responses to islet cell autoantigens, which precede the clinical onset of the disease process. The immunological diagnosis of autoimmune diseases relies primarily on the detection of autoantibodies in the serum of T1DM patients. Although their pathogenic significance remains uncertain, they have the practical advantage of serving as surrogate biomarkers for predicting the clinical onset of T1DM. Type 1 diabetes is a polygenic disease association with specific gene such as HLA gene with a large number of genes having high effects on T1DM pathogenesis. Risk of T1DM progression is conferred by specific allele of HLA gene (DQB1* 0201).
Molecular Detection of Some Mutations Associated with Βeta-Thalassaemia in Iraq
Finally, mutations within families, pointed to a positive correlation between the types of mutations in sons or daughters and their fathers and/or mothers; this indicates the accuracy of the ARMS technique in detection of β-thalassaemia mutations. This conclusion should be taken with caution due to the limited number of families.
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